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          可發酵糖為底物不同菌種丁醇生產能力的研究

          來源: 作者: 編輯: 時間:2012-08-23

          【作者】 過泳安; 滕雅群; 諸歐浩迪; 戴漪晨; 査晶晶; 朱旭; 曾曉; 邢曉雪; Mitchell Bieniek; Garrett Flack; 呂繼華;

          【Author】 GUO Yong-an1 TENG Ya-qun1 ZHU Ouhaodi1 DAU Yi-chen1 ZHA Jing-jing1 ZHU Xu1 ZENG Xiao1 XING Xiao-xue1 Mitchell Bieniek2 Garrett Flack2 LV Ji-hua1(1 The High School Affiliated to Renmin University of China,Beijing 100080,China)(2 Illinois Mathematics and Science Academy,Illinois 60506,U.S.A)

          【機構】 中國人民大學附屬中學; 美國伊利諾伊理科高中;

          【摘要】 隨著新一代生物質能源的研發,利用梭菌的發酵生產丁醇已成為熱點。選用能生產丁醇的Clostridium acetobutylicum AS1.7,Clostridium acetobutylicum AS1.132,Clostridium acetobutylicumAS1.134和Clostridium beijerinckii NCMIB 8052,在多種糖源下進行發酵培養,通過比較其在不同糖源條件下的生長情況、糖利用率、丁醇及副產物產量、對丁醇、木糖耐受能力等,綜合篩選出了最適用于發酵生產丁醇的備選菌種。NCMIB8052因具有最高產量、相對優良的耐受性及可利用多種糖源的特點,而被確定為發酵能力最強的菌種。

          【Abstract】 With the development of the new generation of biomass energy,it has become a popular topic that to use fermentable sugar and Clostridia to produce butanol.The study is going to use Clostridium acetobutylicum AS1.7,Clostridium acetobutylicum AS1.132,Clostridium acetobutylicum AS1.134 and Clostridium beijerinckii NCMIB 8052,which can produce butanol in various sources of sugar by fermentation.By comparing growth behaviors,ratio of using sugars,yield of butanol and by-product,and endurance of butanol and xylose,we are going to find out the most suitable Clostridium,which can be used to the industrial.In the experiment,NCMIB8052 is the most outstanding Clostridium as it has the highest yield,relative high endurance and the ability using multiple sugars.

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